As DNA sequencing becomes cheaper and more prevalent, databases like ClinVar are a valuable resource for elucidating how genomic variability relates to disease progression. This research will use this database to generate clinically curated kinase mutants in order to determine if a change in kinase activity has occurred. Further analysis will be performed to assess the effect of altered kinase activity on cellular function, especially with respect to disease pathogenicity. Additionally, mutagenesis studies will be performed on kinases to probe the sensitivity of their activity to a variety of types of mutations.